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Crystal Violet

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Crystal Violet Basic information

Product Name:
Crystal Violet
Synonyms:
  • PYOKTANIN BLUE
  • S NO 785
  • VIOLET 7B
  • BASIC VIOLET 3
  • BRILLIANT VIOLET
  • CALCOZINE VIOLET 6BN
  • GRAM STAIN NO 1
  • GRAM STAIN
CAS:
548-62-9
MF:
C25H30ClN3
MW:
407.99
EINECS:
208-953-6
Product Categories:
  • Triphenylmethane
  • Amines
  • Colours, Dyes, Indicators & Pigments
  • Aromatics
  • Intermediates & Fine Chemicals
  • Pharmaceuticals
  • Dyes and Pigments
  • Microscopy Reagents and Kits
  • Staining Solutions
  • Staining Solutions for Simple Stains of Bacteria
  • Indicator SolutionsTitration
  • Hematology and Histology
  • Histology Special Stains
  • GVS
  • Analytical Chemistry
  • Indicator (pH)
  • Ion Association
  • pH Indicators
  • G-H-I
  • Stains and Dyes
  • Stains&Dyes, A to
  • Indicators
  • Indicators for non-aqueous titrations
  • Titration
  • 548-62-9
Mol File:
548-62-9.mol
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Crystal Violet Chemical Properties

Melting point:
205 °C (dec.) (lit.)
Boiling point:
560.86°C (rough estimate)
Density 
1.19 g/cm3 at 20 °C
vapor pressure 
0Pa at 25℃
refractive index 
1.6010 (estimate)
Flash point:
40 °C
storage temp. 
room temp
solubility 
water: soluble50g/L at 27°C
pka
9.4(at 25℃)
Colour Index 
42555
form 
Solid
color 
S. No.: 785
PH
2.5-3.5 (10g/l, H2O, 20℃)
PH Range
0.8(yellow)-2.6(blue/violet)
Odor
Slight characteristic odor
Water Solubility 
16 g/L (25 ºC)
λmax
590nm
Merck 
14,4395
BRN 
4077708
Stability:
Stable. Incompatible with strong oxidizing agents, strong acids. Light-sensitive. Combustible.
Biological Applications
Detecting microorganisms; treating atopic dermatitis,dermatological diseases,28,skin wounds,lesions,hemorrhoids,1,multiple myeloma,Non-Hodgkin’s lymphoma,breast cancer,neurodegenerative diseases,onychomycosis; wound dressing
Major Application
Photoresists, lithographic printing plate, printed circuit board, inks, hair dyes, shampoo, drug screening method, bone cement preparation method, microorganisms, hemorrhoids, antifungal, antibacterial, antimalarial agent, dental application
InChIKey
ZXJXZNDDNMQXFV-UHFFFAOYSA-M
LogP
1.172 at 25℃
CAS DataBase Reference
548-62-9(CAS DataBase Reference)
EPA Substance Registry System
Crystal violet (548-62-9)
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Safety Information

Hazard Codes 
Xn,N,T,C
Risk Statements 
52/53-50/53-45-41-22-40-34-10-36/37/38-23/25-51/53-68
Safety Statements 
61-60-53-45-46-36/37/39-26-39-36/37-16-22
RIDADR 
UN 3077 9/PG 3
WGK Germany 
3
RTECS 
BO9000000
TSCA 
Yes
HazardClass 
9
PackingGroup 
III
HS Code 
32041300
Hazardous Substances Data
548-62-9(Hazardous Substances Data)
Toxicity
LD50 orally in mice, rats: 1.2, 1.0 g/kg (Hodge)

MSDS

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Crystal Violet Usage And Synthesis

Physical and Chemical Properties

Crystal Violet is also known as "purple", "methyl violet", "gentian violet." It belongs to triphenylmethane type alkaline dyes. Scientific name:"hexamethyl chloride rose aniline." It appears as dark green powder with metallic luster. It is soluble in water, alcohol and chloroform, but insoluble in ether. Its solubility in water is 1.68% and its solubility in 95% ethanol is 13.87%. However, the solubility of its iodide in two solvents is only 0.035% and 1.78% respectively. Both its aqueous solution and alcohol solution are purple. The structural formula of its chloride is:

Application

Crystal Violet can be used as the dyes of silk, paper and acrylic as well as biological stains. It can be used for manufacturing paints and printing inks. It can also used as an acid-alkaline indicator with coloring range being from pH 0.5 (green) to 2.0 (blue), and developing reagent for colorimetric assays.
It can form a colored chelate with thallium in the hydrobromic acid medium, thus it can be used as thallium sensitivity reagent. It can also be used for the determination of other metal ions such as zinc, antimony, titanium, cadmium, tungsten, gold, mercury and so on. It can also be used as biological stains and non-aqueous titration acid-base indicator. In addition, it can also be used as antiseptic for inhibiting Staphylococcus aureus, Streptococcus and some fungi. Its 1% aqueous solution is commonly known as "purple syrup" for the prevention and treatment of skin and mucous membrane infections. Its enteric-coated tablets can be used as orally anti-pinworm medicine.

Description

Crystal Violet is light sensitive. May react vigorously with strong oxidizing agents. May react exothermically with reducing agents to release gaseous hydrogen.

Description

Anti-infective (topical). Has been used as anthelmintic (Nematodes), as indicator for copper salts.

Chemical Properties

dark green powder or crystals

Chemical Properties

This is a perennial, herbaceous plant native to mountainous areas of Europe; it may reach 0.5 to 1.0 m (2 to 3 ft) in height It has large, cylindrical roots (internally yellow); erect chalice; and fowers with a yellow corolla and peduncles It blooms from July to August The part used is the root of two-year-old plants The color of the rhizomes ranges from dark brown to light tan The color of roots appears to be related to its bitter principle content The dried product and its derivatives of gentian exhibit a very bitter favor.

Occurrence

Gentian is a fl owering perennial found in Europe and Asia.

Uses

Crystal Violet is used as an acid-base indicator, an alcohol denaturant, a biological stain, a textile dye and an indicator for copper salts. It is a component of gram staining in microorganisms and cell lines.

Uses

Gentian Violet is the primary stain used in the Gram stain technique for differentiation of Gram-negative versus Gram-postive bacteria. Used as a component of CV cytotoxicity assays.

Uses

As dye for wood, silk, paper; in inks; as biological stain.

Definition

ChEBI: Crystal violet is an organic chloride salt that is the monochloride salt of crystal violet cation. It has been used in creams for the topical treatment of bacterial and fungal infections, being effective against some Gram-positive bacteria (notably Staphylococcus species) and some pathogenic fungi (including Candida species) but use declined following reports of animal carcinogenicity. It has also been used for dying wood, silk, and paper, as well as a histological stain. It has a role as a histological dye, an antiseptic drug, an antibacterial agent, an antifungal agent and an anthelminthic drug. It contains a crystal violet cation.

Preparation

It is manufactured through using N, N-dimethylaniline as raw materials, followed by condensation, addition, chlorination and other reactions. Alternatively, it can be synthesized through the reaction between Michler ketone and N, N-dimethylaniline reaction in the presence of phosphorus oxychloride, followed by azeotropic reaction with hydrochloric acid. Recrystallization in hot water will generate compound containing nine crystal water molecules.

brand name

Genapax (Key); Gvs (Savage).

Essential oil composition

A number of bitter compounds present in gentian are primarily amarogentin (strongly bitter), gentiopricin (approximately 1.5% in fresh root), swertiamarin and gentiopricroside. The leaves and flowers contain mainly xanthones. Secoiridoids and flavonoids were also detected. In the phase of flowering, leaves are rich with compounds possessing C-glycoside structures while O-glycoside structures accumulate mainly before flowering.

General Description

It belongs to triamino-triphenylmethane synthetic dyes; alkaline. It is an important dye used in bacterial Gram stain. The mixed reagent of crystal violet chloride and iodine is known as gentian violet.

Air & Water Reactions

Insoluble in water.

Fire Hazard

Flash point data for Crystal Violet are not available, however, Crystal Violet is probably combustible.

Flammability and Explosibility

Not classified

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Clinical Use

Gentian violet is variously known as hexamethyl-p-rosanilinechloride, crystal violet, methyl violet, and methylrosanilinechloride. It occurs as a green powder or greenflakes with a metallic sheen. The compound is soluble inwater (1:35) and alcohol (1:10) but insoluble in nonpolar organicsolvents. Gentian violet is available in vaginal suppositoriesfor the treatment of yeast infections. It is also used asa 1% to 3% solution for the treatment of ringworm and yeastinfections. Gentian violet has also been used orally as an anthelminticfor strongyloidiasis (threadworm) and oxyuriasis.

Safety Profile

Poison by ingestion, intravenous, and intraperitoneal routes, An experimental teratogen. Other experimental reproductive effects. A human sktn irritant. Human mutation data reported. Questionable carcinogen with experimental carcinogenic data. When heated to decomposition it emits very toxic fumes of NO, and Cl-.

storage

Room temperature

Properties and Applications

TEST ITEMS

SPECIFICATION

APPEARANCE

DARK GREEN POWDER OR GRANULE

SHADE

BLUISH

HEAT RESISTANCE

200 °C min

DENSITY

1.07 g/cm 3

WATER FASTNESS

4-5

LIGHT FASTNESS

1-2

BLEACHABILITY (OXIDATIVE)

4

BLEACHABILITY (REDUCTIVE)

3

WATER SOLUBILITY AT 25 °C

16 g/L min

WATER INSOLUBLE

1.0% max

MOISTURE

3.0% max

TINTING STRENGTH

100-105 %

WEIGHT METAL TOTAL

50ppm max

Purification Methods

Crystallise the dye from water (20mL/g), the crystals being separated from the chilled solution by centrifugation, then wash them with chilled EtOH (solubility is 1g in 10 mL of hot EtOH) and diethyl ether and dry under vacuum. It is soluble in CHCl3 but insoluble in Et2O. The carbinol is precipitated from an aqueous solution of the dye-hydrochloride, using excess NaOH, then dissolve in HCl and recrystallise it from water as the chloride [UV and kinetics: Turgeon & La Mer J Am Chem Soc 74 5988 1952]. The carbinol base has m 195o (needles from EtOH). The diphthalate (blue and turns red in H2O) crystallises from H2O, m 153-154o(dec at 185-187o)[Chamberlain & Dull J Am Chem Soc 50 3089 1928]. [Beilstein 13 H 233, 13 IV 2284.]

Crystal VioletSupplier

Shenyang Snota Biotech Co.,Ltd Gold
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+86-024-31174117 +86-18002429090
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HENGSHUI HAOYE CHEMICAL CO.,LTD. Gold
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J & K SCIENTIFIC LTD.
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010-82848833 400-666-7788
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Meryer (Shanghai) Chemical Technology Co., Ltd.
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4006608290; 18621169109
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3B Pharmachem (Wuhan) International Co.,Ltd.
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821-50328103-801 18930552037
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3bsc@sina.com