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Crystal Violet

Basic information Physical and Chemical Properties Application Safety Supplier Related

Crystal Violet Basic information

Product Name:
Crystal Violet
Synonyms:
  • PYOKTANIN BLUE
  • S NO 785
  • VIOLET 7B
  • BASIC VIOLET 3
  • BRILLIANT VIOLET
  • CALCOZINE VIOLET 6BN
  • GRAM STAIN NO 1
  • GRAM STAIN
CAS:
548-62-9
MF:
C25H30ClN3
MW:
407.99
EINECS:
208-953-6
Product Categories:
  • Triphenylmethane
  • Amines
  • Aromatics
  • Intermediates & Fine Chemicals
  • Pharmaceuticals
  • Colours, Dyes, Indicators & Pigments
  • Dyes and Pigments
  • GVS
  • Microscopy Reagents and Kits
  • Staining Solutions
  • Staining Solutions for Simple Stains of Bacteria
  • Hematology and Histology
  • Histology Special Stains
  • Indicator SolutionsTitration
  • Indicators
  • Indicators for non-aqueous titrations
  • Titration
  • G-H-I
  • Stains and Dyes
  • Stains&Dyes, A to
  • Analytical Chemistry
  • Indicator (pH)
  • Ion Association
  • pH Indicators
Mol File:
548-62-9.mol
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Crystal Violet Chemical Properties

Melting point:
205 °C (dec.)(lit.)
Boiling point:
560.86°C (rough estimate)
Density 
1.19 g/cm3 (20℃)
refractive index 
1.6010 (estimate)
Flash point:
40 °C
storage temp. 
Store at RT.
solubility 
water: soluble50g/L at 27°C
pka
9.4(at 25℃)
Colour Index 
42555
form 
Solid
color 
S. No.: 785
Odor
Slight characteristic odor
PH
2.5-3.5 (10g/l, H2O, 20℃)
PH Range
0.8(yellow)-2.6(blue/violet)
Water Solubility 
16 g/L (25 ºC)
λmax
590nm
Merck 
14,4395
BRN 
4077708
Stability:
Stable. Incompatible with strong oxidizing agents, strong acids. Light-sensitive. Combustible.
Biological Applications
Detecting microorganisms; treating atopic dermatitis,dermatological diseases,28,skin wounds,lesions,hemorrhoids,1,multiple myeloma,Non-Hodgkin’s lymphoma,breast cancer,neurodegenerative diseases,onychomycosis; wound dressing
Major Application
Photoresists, lithographic printing plate, printed circuit board, inks, hair dyes, shampoo, drug screening method, bone cement preparation method, microorganisms, hemorrhoids, antifungal, antibacterial, antimalarial agent, dental application
InChIKey
ZXJXZNDDNMQXFV-UHFFFAOYSA-M
CAS DataBase Reference
548-62-9(CAS DataBase Reference)
EPA Substance Registry System
Crystal violet (548-62-9)
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Safety Information

Hazard Codes 
Xn,N,T,C
Risk Statements 
52/53-50/53-45-41-22-40-34-10-36/37/38-23/25-51/53-68
Safety Statements 
61-60-53-45-46-36/37/39-26-39-36/37-16-22
RIDADR 
UN 3077 9/PG 3
WGK Germany 
3
RTECS 
BO9000000
TSCA 
Yes
HazardClass 
9
PackingGroup 
III
HS Code 
32041300
Hazardous Substances Data
548-62-9(Hazardous Substances Data)
Toxicity
LD50 orally in mice, rats: 1.2, 1.0 g/kg (Hodge)

MSDS

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Crystal Violet Usage And Synthesis

Physical and Chemical Properties

It is also known as "purple", "methyl violet", "gentian violet." It belongs to triphenylmethane type alkaline dyes. Scientific name:"hexamethyl chloride rose aniline." It appears as dark green powder with metallic luster. It is soluble in water, alcohol and chloroform, but insoluble in ether. Its solubility in water is 1.68% and its solubility in 95% ethanol is 13.87%. However, the solubility of its iodide in two solvents is only 0.035% and 1.78% respectively. Both its aqueous solution and alcohol solution are purple. The structural formula of its chloride is:

Application

It can be used as the dyes of silk, paper and acrylic as well as biological stains. It can be used for manufacturing paints and printing inks. It can also used as an acid-alkaline indicator with coloring range being from pH 0.5 (green) to 2.0 (blue), and developing reagent for colorimetric assays.
It can form a colored chelate with thallium in the hydrobromic acid medium, thus it can be used as thallium sensitivity reagent. It can also be used for the determination of other metal ions such as zinc, antimony, titanium, cadmium, tungsten, gold, mercury and so on. It can also be used as biological stains and non-aqueous titration acid-base indicator. In addition, it can also be used as antiseptic for inhibiting Staphylococcus aureus, Streptococcus and some fungi. Its 1% aqueous solution is commonly known as "purple syrup" for the prevention and treatment of skin and mucous membrane infections. Its enteric-coated tablets can be used as orally anti-pinworm medicine.

Description

Crystal Violet is light sensitive. May react vigorously with strong oxidizing agents. May react exothermically with reducing agents to release gaseous hydrogen.

Description

Anti-infective (topical). Has been used as anthelmintic (Nematodes), as indicator for copper salts.

Chemical Properties

dark green powder or crystals

Chemical Properties

This is a perennial, herbaceous plant native to mountainous areas of Europe; it may reach 0.5 to 1.0 m (2 to 3 ft) in height It has large, cylindrical roots (internally yellow); erect chalice; and fowers with a yellow corolla and peduncles It blooms from July to August The part used is the root of two-year-old plants The color of the rhizomes ranges from dark brown to light tan The color of roots appears to be related to its bitter principle content The dried product and its derivatives of gentian exhibit a very bitter favor.

Occurrence

Gentian is a fl owering perennial found in Europe and Asia.

Uses

As dye for wood, silk, paper; in inks; as biological stain.

Preparation

It is manufactured through using N, N-dimethylaniline as raw materials, followed by condensation, addition, chlorination and other reactions. Alternatively, it can be synthesized through the reaction between Michler ketone and N, N-dimethylaniline reaction in the presence of phosphorus oxychloride, followed by azeotropic reaction with hydrochloric acid. Recrystallization in hot water will generate compound containing nine crystal water molecules.

brand name

Genapax (Key); Gvs (Savage).

Essential oil composition

A number of bitter compounds present in gentian are primarily amarogentin (strongly bitter), gentiopricin (approximately 1.5% in fresh root), swertiamarin and gentiopricroside. The leaves and flowers contain mainly xanthones. Secoiridoids and flavonoids were also detected. In the phase of flowering, leaves are rich with compounds possessing C-glycoside structures while O-glycoside structures accumulate mainly before flowering.

General Description

It belongs to triamino-triphenylmethane synthetic dyes; alkaline. It is an important dye used in bacterial Gram stain. The mixed reagent of crystal violet chloride and iodine is known as gentian violet.

Air & Water Reactions

Insoluble in water.

Fire Hazard

Flash point data for Crystal Violet are not available, however, Crystal Violet is probably combustible.

Clinical Use

Gentian violet is variously known as hexamethyl-p-rosanilinechloride, crystal violet, methyl violet, and methylrosanilinechloride. It occurs as a green powder or greenflakes with a metallic sheen. The compound is soluble inwater (1:35) and alcohol (1:10) but insoluble in nonpolar organicsolvents. Gentian violet is available in vaginal suppositoriesfor the treatment of yeast infections. It is also used asa 1% to 3% solution for the treatment of ringworm and yeastinfections. Gentian violet has also been used orally as an anthelminticfor strongyloidiasis (threadworm) and oxyuriasis.

Safety Profile

Poison by ingestion, intravenous, and intraperitoneal routes, An experimental teratogen. Other experimental reproductive effects. A human sktn irritant. Human mutation data reported. Questionable carcinogen with experimental carcinogenic data. When heated to decomposition it emits very toxic fumes of NO, and Cl-.

Purification Methods

Crystallise the dye from water (20mL/g), the crystals being separated from the chilled solution by centrifugation, then wash them with chilled EtOH (solubility is 1g in 10 mL of hot EtOH) and diethyl ether and dry under vacuum. It is soluble in CHCl3 but insoluble in Et2O. The carbinol is precipitated from an aqueous solution of the dye-hydrochloride, using excess NaOH, then dissolve in HCl and recrystallise it from water as the chloride [UV and kinetics: Turgeon & La Mer J Am Chem Soc 74 5988 1952]. The carbinol base has m 195o (needles from EtOH). The diphthalate (blue and turns red in H2O) crystallises from H2O, m 153-154o(dec at 185-187o)[Chamberlain & Dull J Am Chem Soc 50 3089 1928]. [Beilstein 13 H 233, 13 IV 2284.]

Crystal VioletSupplier

J & K SCIENTIFIC LTD.
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