URICASE Chemical Properties

storage temp. 

-20°C

form 

lyophilized powder

color 

White to off-white

biological source

Bacillus sp. (B. fastidiosus)

Water Solubility 

Hardly soluble in water, slightly soluble in buffer alkaline solution.

Merck 

13,9944

Specific Activity

15-30units/mg protein (biuret)

InChIKey

VPXDHZMLJOJKOX-UHFFFAOYSA-N

EPA Substance Registry System

Oxidase, urate (9002-12-4)

Safety Information

Safety Statements 

24/25

WGK Germany 

3

RTECS 

YU7100000

F 

10-21

TSCA 

Yes

HS Code 

35079090

MSDS

• Language:English Provider:SigmaAldrich

URICASE Usage And Synthesis

Chemical Properties

White powder

Uses

Uricase is very important for the determination of uric acid in biological fluids. Uricase is an important medical enzyme which can be used to determine urate in clinical analysis. The enzyme Urate oxidase (UO) catalyzes the oxidation of uric acid to 5-hydroxyisourate.

Uses

Uricase from Arthrobacter globiformis has been used for coating the electrodes in - biosensor fabrication for the detection of uric acid in urine samples. It has also been used for analysing the effect of Zn, Cu, Mg, and Mn ions on uricase.

Uses

In the determination of serum and urine uric acid.

General Description

Mice deficient in uricase develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment.

Biochem/physiol Actions

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation .

Description

Urate Oxidase Recombinant produced in E.Coli is a tetrameric, non-glycosylated polypeptide chain containing 302 amino acids, having a molecular formula of C1523H2383N417O462S7 and a molecular mass of 34,247 Dalton.
The cDNA coding for urate-oxidase was cloned from a strain of Aspergillus flavus . The monomer protein has no intra- or inter-disulfide bridges.

Source

Escherichia Coli

Background

Urate oxidase catalyzes the enzymatic oxidation (degrades) of uric acid into allantoin, an inactive and soluble metabolite, which is 5 to 10 fold more soluble than uric acid . Urate oxidase is an enzyme of the purine breakdown pathway that catalyses the oxidation of uric acid to allantoin. Uricase is present in numerous diverse organisms, but not in higher primates including human. Hyperuricaemia is most commonly associated with gout and also occurs in mammalians with malignancy, especially those with lymphoid malignancies due to rapid cell turnover and an increased rate of purine metabolism. Uricase is effective in the prevention and treatment of hyperuricaemia in mammalians with malignancy and in those who have undergone transplantation. It appears to act rapidly, safely and induces a more dramatic decrease in plasma levels of uric acid.

URICASE(9002-12-4)Related Product Information

• PHENYLSELENOL
• COBALT(II) ACETYLACETONATE
• Ethyl isocyanoacetate
• Cupric acetylacetonate
• Tosylmethyl isocyanide
• Aluminum acetylacetonate
• 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
• METHYL ISOCYANOACETATE
• 2,4-PENTANEDIONE, SILVER DERIVATIVE
• DICHLORO(ETHYLENEDIAMINE)PLATINUM(II)
• N-BUTYLISOCYANIDE
• Benzyl isocyanide
• Tris(2,4-pentanedionato)chroMiuM(III)
• SALCOMINE
• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III)
• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III)
• Ferric acetylacetonate
• Uric acid