EC 2.6.1.2 Chemical Properties

storage temp. 

-70°C

form 

suspension (aqueous)

color 

green-yellow

biological source

Porcine heart

Water Solubility 

Dissolves readily at 5 mg/ml in 0.05M potassium phosphate pH 7.5 to give a clear, pale-green solution. Soluble in distilled water or dilute buffer.

BRN 

3562140

Specific Activity

≥75units/mg protein

InChI

InChI=1S/C11H16N2O6/c1-5-3-13(11(18)12-10(5)17)8-2-6(15)9(16)7(4-14)19-8/h3,6-9,14-16H,2,4H2,1H3,(H,12,17,18)

InChIKey

VVJYUAYZJAKGRQ-UHFFFAOYSA-N

SMILES

C1(=O)N(C2CC(O)C(O)C(CO)O2)C=C(C)C(=O)N1

EPA Substance Registry System

Aminotransferase, alanine (9000-86-6)

Safety Information

WGK Germany 

3

F 

10

TSCA 

Yes

MSDS

• Language:English Provider:SigmaAldrich

EC 2.6.1.2 Usage And Synthesis

Uses

It is used for research and clinical diagnostic assays for liver disease. Alanine transaminase (GPT/ALT) is used in multi-analyte clinical chemistry controls and calibrators for major IVD manufacturers. The alanine aminotransferase (ALT) blood test is typically used to detect liver injury. Some of the other applications include Diagnostic Controls, calibrators & standards, immunoassays, clinical chemistry, testing/assay validation, life Science, manufacturing, validation Studies.

General Description

Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.

Biochem/physiol Actions

Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine. It plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.

Description

GPT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 516 amino acids (1-496) and having a molecular mass of 56.8 kDa.
GPT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Ecoli

Background

GPT catalyzes the reversible transamination between alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT has a crucial part in the intermediary metabolism of glucose and amino acids. GPT is broadly used as an indicator of liver reliability or hepatocellular destruction in clinical tests.

EC 2.6.1.2(9000-86-6)Related Product Information

• Tris(2,4-pentanedionato)chroMiuM(III)
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• Benzyl isocyanide
• Ethyl isocyanoacetate
• TERT-BUTYL ISOCYANIDE
• Cupric acetylacetonate
• PHENYLSELENOL
• Tosylmethyl isocyanide
• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III)
• METHYL ISOCYANOACETATE
• 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III)
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• N-BUTYLISOCYANIDE
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• Ferric acetylacetonate
• Alanine Aminotransferase