β-Glucuronidase Chemical Properties

storage temp. 

-20°C

form 

powder

color 

yellow-brown

biological source

limpet (Patella vulgata)

Water Solubility 

water: soluble

Merck 

13,4480

Specific Activity

5,000,000-20,000,000units/g protein, pH 6.8 (30 min assay)

Safety Information

Hazard Codes 

Xn

Risk Statements 

42/43

Safety Statements 

22-36/37-45-23

WGK Germany 

3

RTECS 

LZ8990000

F 

3-10

Storage Class

10 - Combustible liquids

Hazard Classifications

Resp. Sens. 1

MSDS

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β-Glucuronidase Usage And Synthesis

Chemical Properties

Off-white or yellow powder, easily soluble in water, pI5.1, optimum pH 4.5-5.0.Stability: under the condition of 4 ℃, keep for six months with 10% decrease in vitality; keep for twelve months with 20% decrease in vitality; adding butanol can increase its stability; if there is turbidity, it does not affect the activity. Inhibitors are D-glucuronic acid, D-galacturonic acid and organic peroxides. 1% aqueous solution has an absorption coefficient of 17.0 at 280 nm. it can rapidly hydrolyze steroidal glucosinolates and other glucosinolates, but not α- or β-glucosides. enzyme reaction: β-D-glucuronic acid + H2O ═D-glucuronic acid + alcohol bound.

Uses

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Uses

β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.

Uses

In the determination of urinary steroids and of steroid conjugates in blood.

General Description

β-Glucuronidase from Escherichia coli is similar to human glucuronidase enzyme and corresponds to molecular weight close to 69-71 kDa and has an pH optimum of 6.5-7.5. It belongs to family-2 glycosyl hydrolase and has active site residues glutamic acid 394, tyrosine 468 and glutamic acid 504.

Biochem/physiol Actions

β-Glucuronidase (GUSB) is a lysosomal enzyme, that degrades glucuronate-containing glycosaminoglycans. β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations. Absence of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain.

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