Pyrophosphatase Chemical Properties

storage temp. 

-20°C

form 

powder

color 

off-white

Specific Activity

≥800units/mg protein

Safety Information

WGK Germany 

3

F 

10

MSDS

• Language:English Provider:SigmaAldrich

Pyrophosphatase Usage And Synthesis

Uses

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PP_i + H₂O → 2P_i.
It plays an important role in protein, RNA, and DNA synthesis.

Definition

An inorganic pyrophos- phatase.

General Description

Pyrophosphatase from E coli (E-PPase) has a broader pH optimum and contains four divalent cations per subunit. It comprises 175 amino acids with additional aspartate residue in the active site cavity. Structurally E-PPase is a homohexamer with six identical 20 kDa subunits. Magnesium is a cofactor for E-PPase.

Biochem/physiol Actions

Pyrophosphatase from E coli (E-PPase) is an essential enzyme in yeast and bacteria The active site residues are crucial for binding to magnesium.

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