ChemicalBook > CAS DataBase List > ENTEROKINASE

ENTEROKINASE

Product Name
ENTEROKINASE
CAS No.
9014-74-8
Chemical Name
ENTEROKINASE
Synonyms
REK;ENTK;PRSS7;TMPRSS15;EC 3.4.21.9;ENTEROKINASE;Enterrokinase;ENTEROPEPTIDASE;Serine protease 7;Peptidase, entero-
CBNumber
CB2204181
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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ENTEROKINASE Property

storage temp. 
-20°C
form 
salt-free, lyophilized powder
color 
white
CAS DataBase Reference
9014-74-8
EPA Substance Registry System
Peptidase, entero- (9014-74-8)
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Safety

WGK Germany 
3
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
E0885
Product name
Enterokinase from porcine intestine
Purity
lyophilized powder, ≥100?units/mg protein
Packaging
40units
Price
$154.8
Updated
2024/03/01
Sigma-Aldrich
Product number
E5144
Product name
Enterokinase from bovine intestine
Purity
powder
Packaging
50units
Price
$388
Updated
2024/03/01
Sigma-Aldrich
Product number
E0885
Product name
Enterokinase from porcine intestine
Purity
lyophilized powder, ≥100?units/mg protein
Packaging
200units
Price
$1050
Updated
2024/03/01
Sigma-Aldrich
Product number
E0632
Product name
Enterokinase from porcine intestine
Purity
≥0.5?units/mg solid
Packaging
1500Units
Price
$400
Updated
2024/03/01
Sigma-Aldrich
Product number
69066-M
Product name
Recombinant Enterokinase
Packaging
50u
Price
$234
Updated
2024/03/01
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ENTEROKINASE Chemical Properties,Usage,Production

Uses

Typical conditions for fusion protein cleavage:
Adjust the concentration of the fusion protein to 1.5 mg/ml and a pH between 7.0-8.0 with 500 mM Tris-HCl, pH 8.0, 2.0 mM CaCl2, and 1% Tween? 20
Add enterokinase to fusion protein solution at a ratio of ~ 0.02 units per 1 mg fusion protein and mix
Incubate reaction mixture at ~25 °C for 16 hours

Definition

An enzyme found in the small intestine, which converts trypsinogen into trypsin.

General Description

Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.

Biochem/physiol Actions

Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.

ENTEROKINASE Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from ENTEROKINASE manufacturers

Zibo Hangyu Biotechnology Development Co., Ltd
Product
ENTEROKINASE 9014-74-8
Price
US $50.00/kg
Min. Order
10kg
Purity
0.99
Supply Ability
20tons
Release date
2023-10-26
WUHAN FORTUNA CHEMICAL CO., LTD
Product
Enteropeptidase 9014-74-8
Price
US $0.00-0.00/g
Min. Order
1g
Purity
98.0%
Supply Ability
1kg/month
Release date
2021-08-25
Career Henan Chemical Co
Product
Enterokinase 9014-74-8
Price
US $1.00/KG
Min. Order
1KG
Purity
≥98%
Supply Ability
20 tons
Release date
2020-01-09

9014-74-8, ENTEROKINASERelated Search:


  • ENTEROPEPTIDASE
  • ENTEROPEPTIDASE, LIGHT CHAIN
  • ENTEROKINASE
  • ENTEROKINASE, LIGHT CHAIN
  • EC 3.4.21.9
  • enterokinase from porcine intestine
  • Peptidase, entero-
  • ENTEROKINASE, BOVINE
  • ENTEROPEPTIDASE FROM PORCINE INTESTINE LYOPHILIZED POWDER
  • Enteropeptidasefromporcine
  • Enterokinase solution from calf intestine
  • Enterokinase from porcine intestine,Enteropeptidase
  • RECOMBINANT ENTEROKINASE
  • REK
  • ENTK
  • PRSS7
  • TMPRSS15
  • Enteropeptidase catalytic light chain
  • Enteropeptidase non-catalytic heavy chain
  • Serine protease 7
  • Recombinant Bovine Enterokinase(rEK)
  • Native Porcine Enterokinase
  • Enterokinase from bovine intestine, Recombinant
  • Enterokinase from Human, Recombinant
  • Native Calf Enterokinase
  • Native Bovine Enterokinase
  • Bovine TMPRSS15 Protein, His Tag
  • Enterrokinase
  • 9014-74-8
  • 9017-74-8
  • 9014-74-9
  • 2019652
  • Proteolytic Enzymes
  • Proteolytic Enzymes and Substrates
  • Enzymes, Inhibitors, and Substrates
  • Biochemicals and Reagents
  • BioChemical