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Neutral Red

Product Name
Neutral Red
CAS No.
553-24-2
Chemical Name
Neutral Red
Synonyms
Neutral Red;N2,N2,7-TriMethylphenazine-2,8-diaMine hydrochloride;Kernechrot;C.I. 50040;neutralredw;Basic Red 5;c.i.basicred5;michromeno.226;cervenneutralni;cervenzasadita5
CBNumber
CB7853810
Molecular Formula
C15H17ClN4
Formula Weight
288.78
MOL File
553-24-2.mol
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Neutral Red Property

Melting point:
290 °C (dec.) (lit.)
Boiling point:
441.31°C (rough estimate)
Density 
1.1590 (rough estimate)
refractive index 
1.6110 (estimate)
storage temp. 
Store at +5°C to +30°C.
solubility 
H2O: soluble10mg/mL
Colour Index 
50040
pka
6.7, 7.4(at 25℃)
form 
Solid
color 
Very dark green
PH
3.1 (10g/l, H2O, 20℃)
PH Range
6.8(red)-8(yellow)
Odor
Odorless
Water Solubility 
50 g/L
λmax
540nm, 533nm, 544nm, 529nm, 454nm
Merck 
14,6488
BRN 
3918740
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Detecting pathogens,bacterial infections; treating age-related macular degeneration,burns,cancer,diabetes,obesity,fungal infections,viral diseases
Major Application
Fuel cell power generation system, liquid crystal displays, solor cells, sensors, thermochromic materials, coloring wood, detergents, assessment of tobacco smoke, cosmetics, detect bacterial infections, multidrug resistance inhibitors, treatment of burns, endodontic, diabetes, obesity, cancer, age-related macular degeneration, viral diseases
CAS DataBase Reference
553-24-2(CAS DataBase Reference)
EPA Substance Registry System
Neutral Red (553-24-2)
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Safety

Hazard Codes 
Xn,Xi
Risk Statements 
68-36/37/38
Safety Statements 
36/37-37/39-36-26-24/25
WGK Germany 
3
RTECS 
SG1400000
TSCA 
Yes
HS Code 
32041300
Toxicity
LD50 intraperitoneal in mouse: 432mg/kg
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
72210
Product name
Neutral Red
Purity
for microscopy (Bact., Bot., Fl., Hist., Vit.), indicator (pH 6.8-8.0)
Packaging
5g
Price
$32.7
Updated
2024/03/01
Sigma-Aldrich
Product number
1.01369
Product name
Neutral red (C.I. 50040)
Purity
indicator and for microbiology
Packaging
25g
Price
$202
Updated
2024/03/01
Sigma-Aldrich
Product number
1.01369
Product name
Neutral red (C.I. 50040)
Purity
indicator and for microbiology
Packaging
100g
Price
$709
Updated
2024/03/01
Sigma-Aldrich
Product number
1.01376
Product name
Neutral red (C.I. 50040)
Purity
for microscopy Certistain?
Packaging
25g
Price
$149
Updated
2023/01/07
TCI Chemical
Product number
N0315
Product name
Basic Red 5
Packaging
25g
Price
$56
Updated
2024/03/01
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Neutral Red Chemical Properties,Usage,Production

Chemical Properties

dark green powder

Uses

Can be used as a vital stain, to stain living cells.

Uses

Neutral Red is a common histology stain used to determine cell viability. It is also used as indicator for nitrogen determination; pH indicator; Dyes and metabolites.

Definition

ChEBI: Neutral red is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+).

Application

Transition of pH Indicator: pH 6.8 - 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it.

Preparation

(a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture.

General Description

Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.

Biochem/physiol Actions

Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.

Biotechnological Applications

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

storage

+4°C

Staining Procedures

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

Properties and Applications

red light purple and red. Soluble in water is cherry red, soluble in ethanol for product red colour with red fluorescent light brown. The strong sulfuric acid for green, blue for diluted, and then into a product red. The dye solution to join strong sodium hydroxide solution have yellow brown precipitation.

Purification Methods

In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]

Neutral Red Preparation Products And Raw materials

Raw materials

Preparation Products

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Neutral Red Suppliers

Kaiyuan city Shenxin fine chemical co., ltd.
Tel
024-024-22595298 18698863499
Fax
024-22595296
Email
1253960@qq.com
Country
China
ProdList
136
Advantage
58
Shenyang yimoke chemical products co. LTD
Tel
13998106873
Email
297303255@qq.com
Country
China
ProdList
149
Advantage
58
Shenyang Shengyuan chemical industry co., ltd
Tel
13190211560 13190211560
Email
1319074246@qq.com
Country
China
ProdList
95
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
94657
Advantage
76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
021-61259108 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40228
Advantage
62
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
Advantage
81
Beijing HwrkChemical Technology Co., Ltd
Tel
010-89508211 18501085097
Fax
010-89508210
Email
sales.bj@hwrkchemical.com
Country
China
ProdList
8418
Advantage
55
Energy Chemical
Tel
021-021-58432009 400-005-6266
Fax
021-58436166
Email
sales8178@energy-chemical.com
Country
China
ProdList
44688
Advantage
61
Shanghai Hanhong Scientific Co.,Ltd.
Tel
021-54306202 13764082696
Email
info@hanhongsci.com
Country
China
ProdList
42958
Advantage
64
Tangshan Moneide Trading Co., Ltd.
Tel
0315-8309571 15633399667
Fax
+86-315-7726572
Email
sales@moneidechem.com
Country
China
ProdList
704
Advantage
57
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View Lastest Price from Neutral Red manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
Basic Red 5 553-24-2
Price
US $0.00/KG
Min. Order
1KG
Purity
98%min
Supply Ability
30tons/month
Release date
2023-01-10
Jiangsu Boquan Biotechnology Co., Ltd.
Product
Basic Red 5 553-24-2
Price
US $190.00-170.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
1tons
Release date
2023-02-21
Zhuozhou Wenxi import and Export Co., Ltd
Product
Basic Red 5 553-24-2
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-07-11

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