Neutral Red
- Product Name
- Neutral Red
- CAS No.
- 553-24-2
- Chemical Name
- Neutral Red
- Synonyms
- Neutral Red;N2,N2,7-TriMethylphenazine-2,8-diaMine hydrochloride;Kernechrot;C.I. 50040;neutralredw;Basic Red 5;c.i.basicred5;michromeno.226;cervenneutralni;cervenzasadita5
- CBNumber
- CB7853810
- Molecular Formula
- C15H17ClN4
- Formula Weight
- 288.78
- MOL File
- 553-24-2.mol
Neutral Red Property
- Melting point:
- 290 °C (dec.) (lit.)
- Boiling point:
- 441.31°C (rough estimate)
- Density
- 1.1590 (rough estimate)
- refractive index
- 1.6110 (estimate)
- storage temp.
- Store at +5°C to +30°C.
- solubility
- H2O: soluble10mg/mL
- Colour Index
- 50040
- pka
- 6.7, 7.4(at 25℃)
- form
- Solid
- color
- Very dark green
- PH Range
- 6.8(red)-8(yellow)
- PH
- 3.1 (10g/l, H2O, 20℃)
- Odor
- Odorless
- Water Solubility
- 50 g/L
- λmax
- 540nm, 533nm, 544nm, 529nm, 454nm
- Merck
- 14,6488
- BRN
- 3918740
- Stability:
- Stable. Incompatible with strong oxidizing agents.
- Biological Applications
- Detecting pathogens,bacterial infections; treating age-related macular degeneration,burns,cancer,diabetes,obesity,fungal infections,viral diseases
- Major Application
- Fuel cell power generation system, liquid crystal displays, solor cells, sensors, thermochromic materials, coloring wood, detergents, assessment of tobacco smoke, cosmetics, detect bacterial infections, multidrug resistance inhibitors, treatment of burns, endodontic, diabetes, obesity, cancer, age-related macular degeneration, viral diseases
- CAS DataBase Reference
- 553-24-2(CAS DataBase Reference)
- EPA Substance Registry System
- Neutral Red (553-24-2)
Safety
- Hazard Codes
- Xn,Xi
- Risk Statements
- 68-36/37/38
- Safety Statements
- 36/37-37/39-36-26-24/25
- WGK Germany
- 3
- RTECS
- SG1400000
- TSCA
- Yes
- HS Code
- 32041300
- Toxicity
- LD50 intraperitoneal in mouse: 432mg/kg
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
N-Bromosuccinimide Price
- Product number
- 72210
- Product name
- Neutral Red
- Purity
- for microscopy (Bact., Bot., Fl., Hist., Vit.), indicator (pH 6.8-8.0)
- Packaging
- 5g
- Price
- $32.7
- Updated
- 2024/03/01
- Product number
- 1.01369
- Product name
- Neutral red (C.I. 50040)
- Purity
- indicator and for microbiology
- Packaging
- 25g
- Price
- $202
- Updated
- 2024/03/01
- Product number
- 1.01369
- Product name
- Neutral red (C.I. 50040)
- Purity
- indicator and for microbiology
- Packaging
- 100g
- Price
- $709
- Updated
- 2024/03/01
- Product number
- 1.01376
- Product name
- Neutral red (C.I. 50040)
- Purity
- for microscopy Certistain?
- Packaging
- 25g
- Price
- $149
- Updated
- 2023/01/07
- Product number
- N0315
- Product name
- Basic Red 5
- Packaging
- 25g
- Price
- $56
- Updated
- 2024/03/01
Neutral Red Chemical Properties,Usage,Production
Chemical Properties
dark green powder
Uses
Can be used as a vital stain, to stain living cells.
Uses
Neutral Red is a common histology stain used to determine cell viability. It is also used as indicator for nitrogen determination; pH indicator; Dyes and metabolites.
Definition
ChEBI: Neutral red is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+).
Application
Transition of pH Indicator: pH 6.8 - 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it.
Preparation
(a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture.
General Description
Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.
Biochem/physiol Actions
Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.
Biotechnological Applications
Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.
storage
+4°C
Staining Procedures
Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.
Properties and Applications
red light purple and red. Soluble in water is cherry red, soluble in ethanol for product red colour with red fluorescent light brown. The strong sulfuric acid for green, blue for diluted, and then into a product red. The dye solution to join strong sodium hydroxide solution have yellow brown precipitation.
Purification Methods
In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]
Neutral Red Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from Neutral Red manufacturers
- Product
- Basic Red 5 553-24-2
- Price
- US $0.00/KG
- Min. Order
- 1KG
- Purity
- 98%min
- Supply Ability
- 30tons/month
- Release date
- 2023-01-10
- Product
- Basic Red 5 553-24-2
- Price
- US $190.00-170.00/kg
- Min. Order
- 1kg
- Purity
- 99%
- Supply Ability
- 1tons
- Release date
- 2023-02-21
- Product
- Basic Red 5 553-24-2
- Price
- US $15.00-10.00/KG
- Min. Order
- 1KG
- Purity
- 99%+ HPLC
- Supply Ability
- Monthly supply of 1 ton
- Release date
- 2021-07-11