ChemicalBook > CAS DataBase List > Z-VAD-FMK

Z-VAD-FMK

Product Name
Z-VAD-FMK
CAS No.
187389-52-2
Chemical Name
Z-VAD-FMK
Synonyms
CS-2005;CS-1507;Z-VAD-FMK;HGNC:1504;Z-VAD(OME)-FMK;Z-VA(OME)D-FMK;Z-VADC(OME)-FMK;Z-VAD-FMK, >=98%;Z-VAL-ALA-ASP-FMK;CASPASE INHIBITOR I
CBNumber
CB0203438
Molecular Formula
C22H30FN3O7
Formula Weight
467.49
MOL File
Mol file
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Z-VAD-FMK Property

Melting point:
>190°C (dec.)
Boiling point:
732.4±60.0 °C(Predicted)
Density 
1.214±0.06 g/cm3 (20 ºC 760 Torr)
Flash point:
396.7℃
storage temp. 
-20°C
solubility 
DMSO: 20 mg/mL
form 
powder
pka
11.05±0.46(Predicted)
color 
white
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 week.
InChIKey
MIFGOLAMNLSLGH-QOKNQOGYSA-N
SMILES
C(N)(=O)[C@H](C)N(C(=O)[C@H](C(C)C)NC(OCC1=CC=CC=C1)=O)[C@@H](CC(OC)=O)C(=O)CF
CAS DataBase Reference
187389-52-2
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Safety

Safety Statements 
22-24/25
WGK Germany 
3
HS Code 
29145090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
V116
Product name
Z-VAD-FMK
Purity
solid
Packaging
2mg
Price
$814
Updated
2024/03/01
Sigma-Aldrich
Product number
SAB2501669
Product name
Anti-CASP3 antibody produced in goat
Purity
affinity isolated antibody, buffered aqueous solution
Packaging
100μg
Price
$568
Updated
2024/03/01
Sigma-Aldrich
Product number
627610
Product name
Caspase Inhibitor I
Packaging
1mg
Price
$318
Updated
2024/03/01
Sigma-Aldrich
Product number
627610
Product name
Caspase Inhibitor I
Packaging
5mg
Price
$662
Updated
2024/03/01
Cayman Chemical
Product number
14463
Product name
Z-VAD(OMe)-FMK
Purity
≥95%
Packaging
1mg
Price
$100
Updated
2024/03/01
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Z-VAD-FMK Chemical Properties,Usage,Production

Description

Z-VAD-FMK (187389-52-2), cell-permeable, methyl ester form. Potent, irreversible pan-caspase inhibitor. Inhibits caspase activity and apoptosis induction in a variety of cell types (IC50 = 1.5 μM). Active in vivo.

Uses

Z-VAD(OMe)-FMK is a cell-permeable, competitive, and irreversible inhibitor of all caspases. Through this action, it inhibits cleavage of poly(ADP-ribose) polymerase, preventing apoptosis when used at 10-50 μM. It also blocks caspase-mediated apoptosis in vivo. Z-VAD(OMe)-FMK effectively prevents caspase action in inflammasomes.[Cayman Chemical]

Uses

Z-VAD-FMK is a caspase inhibitor that inhibits apoptosis and caspase processing in Jurkat T cells treated with low concentrations of z-FA-CMK.

Definition

ChEBI: Z-Val-Ala-Asp(OMe)-CH2F is a tripeptide consisting of Z-Val-Ala-Asp(OMe) in which the C-terminal OH group has been replaced by a fluoromethyl group. An irreversible pan-caspase inhibitor. It has a role as an apoptosis inhibitor and a protease inhibitor. It is a carbamate ester, a tripeptide and an organofluorine compound.

General Description

A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.

Biological Activity

Cell-permeable, irreversible pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC 50 = 0.0015-5.8 mM). Active in vivo .

Biochem/physiol Actions

Cell permeable: yes

Enzyme inhibitor

This tripeptide halomethyl ketone (FWfree-acid = 467.49 g/mol; Soluble to 9.35 mg/ml in DMSO; CAS RegistryNumber = 187389-52-2), also known as Z-VAD-FMK and caspase inhibitor VI, is a broad-spectrum caspase inhibitor that blocks caspase-mediated apoptosis. Z-VAD-FMK inhibits caspase processing (IC50 = 0.0015–5.8 mM, depending on enzyme and cell type). The aspartate methyl ester derivative (FW = 467.49 g/mol), also known as caspase inhibitor I, is far more cell-permeable. Target (s) : caspases; caspase-1; caspase-2; caspase-3; caspase-4 ; caspase 5; caspase-6; caspase-7; caspase-8 ; caspase-9; caspase-10.

storage

-20°C

References

1) Slee et al. (1996), Benzyloxycarbonyl-Val-Ala-ASP (OMe) fluoromethylketone (Z-VAD-FMK) inhibits apoptosis by blocking the processing of CPP32; Biochem. J., 315 21 2) Kunstle et al. (1997), ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-alpha; Immunol. Lett., 55 5 3) Garcia-Calvo et al. (1998), Inhibition of human caspases by peptide based and macromolecular inhibitors; J. Biol. Chem., 273 32608

Z-VAD-FMK Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Z-VAD-FMK manufacturers

Henan Aochuang Chemical Co.,Ltd.
Product
Z-VAD-FMK 187389-52-2
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
98%
Supply Ability
1ton
Release date
2022-10-08
Career Henan Chemical Co
Product
Z-VAD-FMK 187389-52-2
Price
US $1.00/KG
Min. Order
1G
Purity
98%
Supply Ability
100KG
Release date
2018-08-21

187389-52-2, Z-VAD-FMKRelated Search:


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