ENTEROKINASE

Product Name: ENTEROKINASE
CAS No.: 9014-74-8
Chemical Name: ENTEROKINASE
Synonyms: REK;ENTK;PRSS7;TMPRSS15;EC 3.4.21.9;ENTEROKINASE;Enterrokinase;ENTEROPEPTIDASE;Serine protease 7;Peptidase, entero-
CBNumber: CB2204181
Molecular Formula: NULL
Formula Weight: 0
MOL File: Mol file

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ENTEROKINASE Property

storage temp.: -20°C
form: salt-free, lyophilized powder
color: white
CAS DataBase Reference: 9014-74-8
EPA Substance Registry System: Peptidase, entero- (9014-74-8)

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Safety

WGK Germany: 3

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: E0885
Product name: Enterokinase from porcine intestine
Purity: lyophilized powder, ≥100?units/mg protein
Packaging: 40units
Price: $154.8
Updated: 2024/03/01

Sigma-Aldrich

Product number: E5144
Product name: Enterokinase from bovine intestine
Purity: powder
Packaging: 50units
Price: $388
Updated: 2024/03/01

Sigma-Aldrich

Product number: E0885
Product name: Enterokinase from porcine intestine
Purity: lyophilized powder, ≥100?units/mg protein
Packaging: 200units
Price: $1050
Updated: 2024/03/01

Sigma-Aldrich

Product number: E0632
Product name: Enterokinase from porcine intestine
Purity: ≥0.5?units/mg solid
Packaging: 1500Units
Price: $400
Updated: 2024/03/01

Sigma-Aldrich

Product number: 69066-M
Product name: Recombinant Enterokinase
Packaging: 50u
Price: $234
Updated: 2024/03/01

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ENTEROKINASE Chemical Properties,Usage,Production

Uses

Typical conditions for fusion protein cleavage:
Adjust the concentration of the fusion protein to 1.5 mg/ml and a pH between 7.0-8.0 with 500 mM Tris-HCl, pH 8.0, 2.0 mM CaCl_2,and 1% Tween^? 20
Add enterokinase to fusion protein solution at a ratio of ~ 0.02 units per 1 mg fusion protein and mix
Incubate reaction mixture at ~25 °C for 16 hours

Definition

An enzyme found in the small intestine, which converts trypsinogen into trypsin.

General Description

Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.

Biochem/physiol Actions

Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.

ENTEROKINASE Preparation Products And Raw materials

Raw materials

Preparation Products

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ENTEROKINASE Suppliers

China 112 Germany 1 India 1 Switzerland 1 United Kingdom 1 United States 14 Global 130

Aladdin Scientific

Tel: +1-+1(833)-552-7181
Email: sales@aladdinsci.com
Country: United States
ProdList: 57505
Advantage: 58

Creative Enzymes

Tel: 1-516-855-7709
Fax: 1-631-938-8127
Email: info@creative-enzymes.com
Country: United States
ProdList: 8748
Advantage: 58

Creative Enzymes

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Country: United States
ProdList: 6057
Advantage: 58

Riedel-de Haen AG

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Country: United States
ProdList: 6773
Advantage: 87

Waterstone Technology, LLC

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Email: sales@waterstonetech.com
Country: United States
ProdList: 6786
Advantage: 30

EMD Biosciences, Inc.

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Email: technical@calbiochem.com
Country: United States
ProdList: 6529
Advantage: 66

Crescent Chemical Co., Inc.

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Country: United States
ProdList: 4597
Advantage: 68

Covance Research Products Inc.

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Country: United States
ProdList: 182
Advantage: 81

Stratagene

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Country: United States
ProdList: 1139
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EMD Biosciences, Inc.

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Email: novatech@novagen.com
Country: United States
ProdList: 609
Advantage: 77

Pfaltz & Bauer, Inc.

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Country: United States
ProdList: 6828
Advantage: 72

Roche Applied Science

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Country: United States
ProdList: 696
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Kamiya Biomedical Company

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Email: ordering@kamiyabiomedical.com
Country: United States
ProdList: 736
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Biozyme Laboratories International, Ltd.

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Email: bioinfo@biozyme.com
Country: United States
ProdList: 60
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View Lastest Price from ENTEROKINASE manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD

Product: Enteropeptidase 9014-74-8
Price: US $0.00-0.00/g
Min. Order: 1g
Purity: 98.0%
Supply Ability: 1kg/month
Release date: 2021-08-25

Zibo Hangyu Biotechnology Development Co., Ltd

Product: ENTEROKINASE 9014-74-8
Price: US $50.00/kg
Min. Order: 10kg
Purity: 0.99
Supply Ability: 20tons
Release date: 2023-10-26

Career Henan Chemical Co

Product: Enterokinase 9014-74-8
Price: US $1.00/KG
Min. Order: 1KG
Purity: ≥98%
Supply Ability: 20 tons
Release date: 2020-01-09

9014-74-8, ENTEROKINASERelated Search:

Proteinase K Aprotinin Trypsin Chymotrypsin METHYL ISOCYANOACETATE SALCOMINE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Ethyl isocyanoacetate TERT-BUTYL ISOCYANIDE 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE PHENYLSELENOL COBALT(II) ACETYLACETONATE 2,4-PENTANEDIONE, SILVER DERIVATIVE N-BUTYLISOCYANIDE Cupric acetylacetonate Aluminum acetylacetonate Tris(2,4-pentanedionato)chroMiuM(III) Tosylmethyl isocyanide

ENTEROPEPTIDASE

ENTEROPEPTIDASE, LIGHT CHAIN

ENTEROKINASE

ENTEROKINASE, LIGHT CHAIN

EC 3.4.21.9

enterokinase from porcine intestine

Peptidase, entero-

ENTEROKINASE, BOVINE

ENTEROPEPTIDASE FROM PORCINE INTESTINE LYOPHILIZED POWDER

Enteropeptidasefromporcine

Enterokinase solution from calf intestine

Enterokinase from porcine intestine,Enteropeptidase

RECOMBINANT ENTEROKINASE

REK

ENTK

PRSS7

TMPRSS15

Enteropeptidase catalytic light chain

Enteropeptidase non-catalytic heavy chain

Serine protease 7

Recombinant Bovine Enterokinase(rEK)

Native Porcine Enterokinase

Enterokinase from bovine intestine, Recombinant

Enterokinase from Human, Recombinant

Native Calf Enterokinase

Native Bovine Enterokinase

Bovine TMPRSS15 Protein, His Tag

Enterrokinase

Recombinant Enterokinase (EK)

9014-74-8

9017-74-8

9014-74-9

2019652

Proteolytic Enzymes

Proteolytic Enzymes and Substrates

Enzymes, Inhibitors, and Substrates

Biochemicals and Reagents

BioChemical