description Content analysis Control of Bacterial Processes Toxicity Utilization limitation Chemical property Uses
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Indole

description Content analysis Control of Bacterial Processes Toxicity Utilization limitation Chemical property Uses
Product Name
Indole
CAS No.
120-72-9
Chemical Name
Indole
Synonyms
1H-INDOLE;Indol;1H-Indol;FEMA 2593;Benzazole;1-BENZAZOLE;Benzopyrrole;BENZO(B)PYRROLE;2,3-BENZOPYRROLE;1H-indole analogue
CBNumber
CB8275772
Molecular Formula
C8H7N
Formula Weight
117.15
MOL File
120-72-9.mol
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Indole Property

Melting point:
51-54 °C (lit.)
Boiling point:
253-254 °C (lit.)
Density 
1.22
vapor pressure 
0.016 hPa (25 °C)
FEMA 
2593 | INDOLE
refractive index 
1.6300
Flash point:
>230 °F
storage temp. 
2-8°C
solubility 
methanol: 0.1 g/mL, clear
form 
Crystalline Powder
pka
3.17 (quoted, Sangster, 1989)
color 
White to slightly pink
PH
5.9 (1000g/l, H2O, 20℃)
Odor
fecal odor, floral in high dilution
Odor Type
animal
Odor Threshold
0.0003ppm
Water Solubility 
2.80 g/L (25 ºC)
Sensitive 
Light Sensitive
Merck 
14,4963
JECFA Number
1301
BRN 
107693
Stability:
Stable, but may be light or air sensitive. Incompatible with strong oxidizing agents, iron and iron salts.
LogP
2.14-2.24
CAS DataBase Reference
120-72-9(CAS DataBase Reference)
NIST Chemistry Reference
Indole(120-72-9)
EPA Substance Registry System
Indole (120-72-9)
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Safety

Hazard Codes 
Xn,N,T
Risk Statements 
21/22-37/38-41-50/53-36-39/23/24/25-23/24/25-52/53
Safety Statements 
26-36/37/39-60-61-45-36/37
RIDADR 
UN 2811 6.1/PG 3
WGK Germany 
1
RTECS 
NL2450000
8-13
TSCA 
Yes
HS Code 
2933 99 20
HazardClass 
9
PackingGroup 
III
Hazardous Substances Data
120-72-9(Hazardous Substances Data)
Toxicity
LD50 orally in rats: 1 g/kg (Smyth)
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H302Harmful if swallowed

H311Toxic in contact with skin

H319Causes serious eye irritation

H400Very toxic to aquatic life

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P273Avoid release to the environment.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
W259306
Product name
Indole
Purity
≥99%, FG
Packaging
100g
Price
$78.7
Updated
2024/03/01
Sigma-Aldrich
Product number
W259306
Product name
Indole
Purity
≥99%, FG
Packaging
1kg
Price
$195
Updated
2024/03/01
Sigma-Aldrich
Product number
W259306
Product name
Indole
Purity
≥99%, FG
Packaging
10Kg
Price
$1300
Updated
2024/03/01
Sigma-Aldrich
Product number
W259306
Product name
Indole
Purity
≥99%, FG
Packaging
25kg
Price
$2890
Updated
2024/03/01
Sigma-Aldrich
Product number
I3408
Product name
Indole
Purity
≥99%
Packaging
25g
Price
$38.2
Updated
2024/03/01
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Indole Chemical Properties,Usage,Production

description

Indole, also called Benzopyrrole, an aromatic heterocyclic organic compound occurring in some flower oils, such as jasmine and orange blossom, in coal tar, and in fecal matter. It has a bicyclic structure, consisting of a six-membered benzene ring fused to a five-membered nitrogen-containing pyrrole ring. It can be produced by bacteria as a degradation product of the amino acid tryptophan. It occurs naturally in human feces and has an intense fecal smell. This off flavour occurs in beer due to contaminant coliform bacteria during the primary fermentation stage of beer brewing. At very low concentrations, however, it has a flowery smell, and is a constituent of many flower scents (such as orange blossoms) and perfumes. Natural jasmine oil, used in the perfume industry, contains around 2.5% of indole. Indole also occurs in coal tar. The participation of the nitrogen lone electron pair in the aromatic ring means that indole is not a base, and it does not behave like a simple amine.
Indoles are important precursors for other substances made within the human body and are, therefore, researched and used in lifestyle and medical applications. The compound was officially discovered in 1866 by a scientist working with the properties of zinc dust who reduced oxindole from the zinc dust into an indole. After the discovery, indoles became important constituents of the textile industry, and as more research was conducted, the larger role that indoles played within the human body system was realized. The indolic nucleus in substances like tryptophan and auxin has led to a better understanding of their mechanism within the body.

Content analysis

Press GT-10-4 gas chromatography method for the determination with polar column.
Using the polar column method in GT-10-4 gas chromatography to determine the content of indole.

Control of Bacterial Processes

As an intercellular signal molecule in both gram-positive and gram-negative bacteria, indole regulates various aspects of bacterial physiology, including spore formation, plasmid stability, resistance to drugs, biofilm formation, and virulence. Indole has been shown to control a number of bacterial processes such as spore formation, plasmid stability, drug resistance, biofilm formation, and virulence. Indole may have anticarcinogenic activity.
Commonly synthesized from phenylhydrazine and pyruvic acid, although several other procedures have been discovered, indole also can be produced by bacteria as a degradation product of the amino acid tryptophan.

Toxicity

GRAS(FEMA)。
LD50 1000 mg/kg(test with rat orally).

Utilization limitation

FEMAmg/kg:soft drinks:0.26;cold drinks:0.28;candy:0.50;bakery product :0.58;pudding:0.02~0.40.
Moderate limit (FDA§172.515,2000)

Chemical property

It is the shiny flaky white crystals, and would turn into dark colors when it exposed to light. There would be a strong unpleasant odor with high concentration of indole, but the flavor would change into oranges and jasmine after highly diluted (concentration <0.1%). It has the melting point of 52~53 ℃ and the boiling point of 253~254 ℃. It is soluble in alcohol, ether, hot water, propylene glycol, petroleum ether and most of the non-volatile oil, insoluble in glycerin and mineral oil.
Natural indole are widely contained in neroli oil, orange oil, lemon oil, lime oil, citrus oil, peel oil, jasmine oil and other essential oil.

Uses

(1) According to the GB 27 60-96 , indole can be used as flavouring agent and mainly used for preparing the essence of cheese, citrus, coffee, nuts, grape, strawberry, raspberry, chocolate, assorted fruit, jasmine and lily etc.
(2) It can be used as the reagent for the determination of nitrite, can also used in the manufacture of perfume and medicine.
(3) It can be used as the raw material of perfume, pharmaceuticals and plant growth hormone.
(4) Indole is the intermediate for the indole acetic acid and indole butyric acid.The indole acetic acid and indole butyric are plant growth regulator.
(5) It can be widely used in the manufacture of the essences of jasmine, lilac, orange blossom, gardenia, honeysuckle, lotus, narcissus, ylang, orchid and prynne etc. It is usually combined with the methyl indole to imitate the artificial civet. The extremely few of the indole can be used in chocolate, raspberry, strawberry, bitter orange, coffee, nuts, cheese, grapes and fruit and other fruity essential oil.
(6) Indole is mainly used as spices, dyes, amino acids and the raw materials of pesticide. Indole itself is a spice commonly used in producing the essences of jasmine, lilac, lotus flowers, orchids and other flower flavor. The usage is generally in a few thousandths.
(7) It can be used for verifying the gold, potassium and nitrite and manufacturing jasmine-type fragrance. It can also be used in pharmaceutical industry.

Description

Indole has an almost floral odor when highly purified. Otherwise, it exhibits the characteristic odor of feces. It is not very stable on exposure to light (turns red). Indole may be obtained from the 220 - 260°C boiling fraction of coal tar or by heating sodium phenylglycine-o-carboxylate with NaOH, saturating the aqueous solution of the melt with C 02, and finally reducing with sodium amalgam; can be prepared also by the reduction of indoxyl, indoxyl carboxylic acid, or indigo.

Chemical Properties

white crystals with an unpleasant odour

Chemical Properties

Indole has an unpleasant odor at high concentration, odor becomes floral at higher dilutions

Physical properties

Colorless to yellow scales with an unpleasant odor. Turns red on exposure to light and air. Odor threshold of 0.14 ppm was reported by Buttery et al. (1988).

Occurrence

Reported occurring in several natural products as a complex compound that decomposes during enfleurage or steam distillation yielding free indole; reported found in the essential oil from flower of Jasminum grandiflorum, in neroli oil and in the oil extracted from flowers of bitter orange; also reported in the flowers of several plants: lemon, coffee, Hevea brasiliensis and Randia formosa in the oil extracted from flowers of Jasminum odoratissinium L. and in the oil of Narcissus jonquilla. Also reported found in apricot, mandarin orange peel oil, grapes, kohlrabi, French fried potato, crispbread, cheeses, butter, milk, milk powder, boiled egg, fish oil, chicken, beef, pork, beer, rum, Finnish whiskey, red and white wine, coffee, tea, soybean, mushrooms, cauliflower, figs, rice, licorice, buckwheat, malt, wort, elder flower, clary sage, shrimp, okra, crab, clam, squid and green maté

Uses

Indole is a flavoring agent that is a white, flaky crystalline product. it has an unpleasant odor when concentrated and a flowery odor when diluted. it is soluble in most fixed oils and propylene glycol and insoluble in glycerin and mineral oil. it is obtained from decomposi- tion of a protein.

Uses

Indole occurs in coal tar. It is used, underhigh dilution, in perfumery, and as an intermediatein organic synthesis.

Uses

In highly dil solutions the odor is pleasant, hence indole has been used in perfumery.

Uses

Can be used in perfumes and in the synthesis of tryptophan.

Definition

indole: A yellow solid, C8H7N, m.p.52°C. Its molecules consist of a benzenering fused to a nitrogen-containingfive-membered ring. It occurs insome plants and in coal tar, and isproduced in faeces by bacterial action.It is used in making perfumes.Indole has the nitrogen atom positionednext to the fused benzenering. An isomer with the nitrogentwo atoms away from the fused ringis called isoindole.

Preparation

Obtained from the 220 to 260°C boiling fraction of coal tar or by heating sodium phenyl-glycine-o-carboxylate with NaOH, saturating the aqueous solution of the melt with CO2 and finally reducing with sodium amalgam; can be prepared also by the reduction of indoxyl, indoxyl carboxylic acid or indigo.

Aroma threshold values

Detection: 140 ppb

Synthesis Reference(s)

The Journal of Organic Chemistry, 55, p. 580, 1990 DOI: 10.1021/jo00289a036
Chemical and Pharmaceutical Bulletin, 35, p. 1823, 1987 DOI: 10.1248/cpb.35.1823

General Description

Indole is classified under the volatile flavor compounds (VFCs). It is known to play significant role in various biological functions such as anti-inflammatory, anticonvulsant, cardiovascular and antibacterial activities.

Hazard

A carcinogen.

Health Hazard

Low to moderate toxicity was observed inexperimental animals resulting from oral orsubcutaneous administration of indole. Theoral LD50 value in rats is 1000 mg/kg. It is ananimal carcinogen. It caused tumors in bloodand lungs in mice subjected to subcutaneousadministration.

Fire Hazard

Noncombustible solid.

Flammability and Explosibility

Not classified

Biochem/physiol Actions

Taste at 0.3-2 ppm

Source

Indole was detected in jasmine flowers (Jasminum officinale), licorice (Glycyrrhiza glabra), kohlrabi stems (Brassica oleracea var. gongylodes), and hyacinth flowers (Hyacinthus orientalis) at concentrations of 42 to 95, 2, 1.33, and 0.24 to 3.45 ppm, respectively. Indole also occurs in tea leaves, black locust flowers, corn leaves, petitgrain, and yellow elder (Duke, 1992).
A liquid swine manure sample collected from a waste storage basin contained indole at a concentration of 4.8 mg/L (Zahn et al., 1997).

Environmental Fate

Biological. In 9% anaerobic municipal sludge, indole degraded to 1,3-dihydro-2H-indol-2-one (oxindole), which degraded to methane and carbon dioxide (Berry et al., 1987). Heukelekian and Rand (1955) reported a 5-d BOD value of 1.70 g/g which is 65.4% of the ThOD value of 2.48 g/g.
Chemical/Physical. The aqueous chlorination of indole by hypochlorite/hypochlorous acid, chlorine dioxide, and chloramines produced oxindole, isatin, and possibly 3-chloroindole (Lin and Carlson, 1984).

Metabolic pathway

The indole is metabolized in a mineral salt medium inoculated with 9% anaerobically digested nitrate- reducing sewage sludge, resulting in the sequential occurrence of four structurally related compounds: oxindole, isatine, dioxindole, and anthranilic acid. Indole is metabolized by fungus via indoxyl (3-hydroxyindole), N-formylanthranilic acid, anthranilic acid, 2,3-dihydroxybenzoic acid, and catecol, which is further degraded by an ortho cleavage.

Metabolism

Indole is oxidized to 3-hydroxyindole (indoxyl) which is conjugated with glucuronic and sulphuric acids before excretion. The sulphate conjugate seems to be the main product in rabbits and, even with relatively large doses of indole, the sulphate conjugation always exceeds that of glucuronic acid(Williams, 1959).

Purification Methods

It can be further purified by sublimation in a vacuum or by zone melting. The picrate forms orange crystals from EtOH and has m 175o. [Beilstein 20 II 196, 20 III/IV 3176, 20/7 V 5.]

Toxicity evaluation

Indole causes oxidative damage to membranes.

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View Lastest Price from Indole manufacturers

Hebei Dangtong Import and export Co LTD
Product
Indole 120-72-9
Price
US $80.00-60.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20Tons
Release date
2023-10-09
Shandong Juchuang Chemical Co., LTD
Product
Indole 120-72-9
Price
US $60.00-40.00/kg
Min. Order
500kg
Purity
99.9
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200tons
Release date
2024-03-11
Hebei Duling International Trade Co. LTD
Product
Indole 120-72-9
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US $30.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
100 tons
Release date
2022-10-13

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  • INDOLE(RG)
  • 1-BENZAZOLE
  • 1-BENZO(B)PYRROLE
  • 2,3-BENZOPYRROLE
  • 1H-BENZO[B]PYRROLE
  • 1H-INDOLE
  • benzo[d]pyrrole
  • INDOLE crystalline extrapure AR
  • ETHYL 4,6-DIAMINO-1H-INDOLE-2-CARBOXYLATE
  • ETHYL 4,6-DINITRO-1H-INDOLE-2-CARBOXYLATE
  • ETHYL 4-AMINO-7-HYDROXY-1H-INDOLE-2-CARBOXYLATE
  • ETHYL 6-AMINO-4-NITRO-1H-INDOLE-2-CARBOXYLATE
  • ETHYL 6-NITRO-4-AMINO-1H-INDOLE-2-CARBOXYLATE
  • ETHYL 7-HYDROXY-4-NITRO-1H-INDOLE-2-CARBOXYLATE
  • INDOL-3-YLACETICACID
  • 3-BROMO-5-METHYL-1H-INDOLE
  • Indole, standard for GC,>99.5%(GC)
  • 1H-indole analogue
  • Indole, 98+%
  • 1H-Indol
  • Benzaole
  • Benzopyrrole
  • Indol
  • Ketole
  • INDOLE, 1000MG, NEAT
  • INDOLE, 99+%
  • INDOLE 99+% FCC
  • INDOLE, 1X1ML, MEOH, 2000UG/ML
  • INDOLE GC STANDARD
  • Benzazole
  • INDOLE (I)
  • Indole perfume
  • Indole industrial
  • Molten indole
  • Indole, (1-Benzazole)
  • Indole, 99+% 100GR
  • Indole, 99+% 25GR
  • Indole GR for analysis
  • 1H-Indole, 1-Azaindene
  • NSC 1964
  • INDOLE GR FOR ANALYSIS 10 G
  • INDOLE GR FOR ANALYSIS 100 G
  • Indole@50 μg/mL in Toluene
  • Indole&gt
  • INDOLE FOR SYNTHESIS 100 G
  • INDOLE FOR SYNTHESIS 25 G