EC 3.4.21.19 Chemical Properties

storage temp. 

-20°C

form 

lyophilized powder

color 

light brown

Water Solubility 

Soluble in water

Safety Information

Hazard Codes 

Xn

Risk Statements 

36/37/38-42/43

Safety Statements 

22-24-26-36/37-45

WGK Germany 

3

RTECS 

TW0175000

F 

10

MSDS

• Language:English Provider:SigmaAldrich

EC 3.4.21.19 Usage And Synthesis

Uses

Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Product P2922 has been used to linearize cyclic peptides in C. ternatea leaf extract.

Uses

Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for:

• obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies.
• the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination.
• limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase).
• the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.

Biochem/physiol Actions

Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.

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