6-chloro-5-(4-(1-hydroxycyclobutyl)phenyl)-1H-indole-3-carboxylicacid
- Product Name
- 6-chloro-5-(4-(1-hydroxycyclobutyl)phenyl)-1H-indole-3-carboxylicacid
- CAS No.
- 1467057-23-3
- Chemical Name
- 6-chloro-5-(4-(1-hydroxycyclobutyl)phenyl)-1H-indole-3-carboxylicacid
- Synonyms
- CS-2760;EOS-61684;PF 6409577;PF-06409577;PF-06409577 ，S8335;PF 06409577;PF06409577;PF-06409577 >=98% (HPLC);PF-06409577, 10 mM in DMSO;PF-06409577;PF 06409577;PF06409577;6-chloro-5-(4-(1-hydroxycyclobutyl)phenyl)-1H-indole-3-carboxylicacid
- CBNumber
- CB33142869
- Molecular Formula
- C19H16ClNO3
- Formula Weight
- 341.79
- MOL File
- 1467057-23-3.mol
6-chloro-5-(4-(1-hydroxycyclobutyl)phenyl)-1H-indole-3-carboxylicacid Property
- Boiling point:
- 610.9±55.0 °C(Predicted)
- Density
- 1.468±0.06 g/cm3(Predicted)
- storage temp.
- room temp
- solubility
- DMSO: soluble
- form
- powder
- pka
- 3.60±0.30(Predicted)
- color
- white to beige
- InChI
- InChI=1S/C19H16ClNO3/c20-16-9-17-14(15(10-21-17)18(22)23)8-13(16)11-2-4-12(5-3-11)19(24)6-1-7-19/h2-5,8-10,21,24H,1,6-7H2,(H,22,23)
- InChIKey
- FHQXLWCFSUSXBF-UHFFFAOYSA-N
- SMILES
- N1C2=C(C=C(C3=CC=C(C4(O)CCC4)C=C3)C(Cl)=C2)C(C(O)=O)=C1
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- PZ0319
- Product name
- PF-06409577
- Purity
- ≥98% (HPLC)
- Packaging
- 5MG
- Price
- $105
- Updated
- 2023/06/20
- Product number
- PZ0319
- Product name
- PF-06409577
- Purity
- ≥98% (HPLC)
- Packaging
- 25MG
- Price
- $424
- Updated
- 2023/06/20
- Product number
- 27366
- Product name
- PF-06409577
- Packaging
- 5mg
- Price
- $100
- Updated
- 2024/03/01
- Product number
- 27366
- Product name
- PF-06409577
- Packaging
- 25mg
- Price
- $343
- Updated
- 2024/03/01
- Product number
- 27366
- Product name
- PF-06409577
- Packaging
- 1mg
- Price
- $32
- Updated
- 2024/03/01
6-chloro-5-(4-(1-hydroxycyclobutyl)phenyl)-1H-indole-3-carboxylicacid Chemical Properties,Usage,Production
Description
PF-06409577 is an activator of AMP-activated protein kinase (AMPK) heterotrimers (Kd = 9 nM for α1β1γ1). In vitro, it selectively activates β1-containing AMPK isoforms (EC50s = 2.18-7.03 nM) over β2-containing isoforms (EC50s = ≥10 μM). PF-06409577 has an EC50 value of 7 nM in an activation/protection TR-FRET assay using α1β1γ1 AMPK. In vivo, PF-06409577 (≥10 mg/kg) increases phosphorylation of AMPK in rat kidney. It decreases urinary albumin levels in a ZSF1 obese rat model of diabetes, metabolic syndrome, and renal disease when administered at a dose of 100 mg/kg.
Uses
PF-06409577 has been used to determine its potential as a therapeutic agent for diabetic nephropathy.
General Description
PF-06409577 is a 6-chloro-indole derivative obtained from 5-bromo-6-chloro-indole.
Biochem/physiol Actions
PF-06409577 preserves retinal pigment epithelium cells from UV radiation by activating AMPK (AMP-activated protein kinase) signaling. This orally bioavailable and possesses pharmacokinetic properties. PF-06409577 blocks de novo lipid and cholesterol synthesis pathways that shows a decrease in hepatic lipids and mRNA expression of markers of hepatic fibrosis.
Synthesis
1467059-92-2
1467057-23-3
Methyl 6-chloro-5-[4-(1-hydroxycyclobutyl)phenyl]-1H-indole-3-carboxylate (131 g, 368 mmol), methanol (2.20 L), and an aqueous sodium hydroxide solution (90.2 g, 2.21 mol, dissolved in 740 mL of water) were added to a round bottom flask, and the reaction was stirred for 18 hours at 70 °C. Upon completion of the reaction, the mixture was cooled to room temperature, filtered through diatomaceous earth, and the filtrate was concentrated to approximately 30% of the original volume, at which time no precipitate formation was observed. After addition of water (700 mL), the clarified tan solution was washed with methyl tert-butyl ether (MTBE, 3 x 250 mL) and the organic layer was discarded. 38% hydrochloric acid (200 mL) was added dropwise to the stirred aqueous phase (total volume of about 1800 mL) to a pH of about 2-3 at 18-25°C, followed by a one-time addition of ethyl acetate (250 mL). Upon addition of ethyl acetate, solids began to precipitate. Heptane (250 mL) was slowly added to the stirred non-homogeneous mixture via an addition funnel at room temperature and stirring was continued for 4 hours. The solid was collected by filtration, washed sequentially with water and ethyl acetate-heptane mixture (1:1), dried by filtration at room temperature and dried under vacuum at 50 °C to give a light yellow solid. The solid was dissolved in tetrahydrofuran (630 mL) and stirred at 65 °C (internal temperature) for 4 h. Ethyl acetate (630 mL) was then slowly added through a dropping funnel and the resulting slurry was slowly cooled at room temperature overnight. The solid was filtered, washed with a tetrahydrofuran-ethyl acetate mixture (1:1) and dried under vacuum at 50 °C to give 107.0 g of product. The mother liquor was concentrated and the residue was washed with acetone, filtered and dried to give another 12.5 g of product. The resulting solid was dissolved in ethanol (800 mL) containing 0.3 mL of 1M NaOH aqueous solution, and water (800 mL) was slowly added through a dosing funnel at 55-60 °C, and a precipitate began to form after the addition of water. The suspension was stirred at 60 °C for 2 h, followed by stirring at 40 °C for 24 h, and finally for 40 h at room temperature. The solid was filtered, washed with an ethanol-water mixture (1:1) and dried under vacuum at 50 °C to afford 6-chloro-5-(4-(1-hydroxycyclobutyl)phenyl)-1H-indole-3-carboxylic acid as an off-white crystalline solid (72.4 g, 58% yield). The mother liquor was concentrated to about 30% of the original volume, precipitate was precipitated, filtered and dried in vacuum to give another batch of product (14.5 g, 12% yield). The total yield was 70%. Mass spectrum (ES-): m/z 340.3 ([M-H]-). 1H NMR (400 MHz, DMSO-d6) δ: 12.12 (s, 1H), 11.95 (br.s, 1H), 8.09 (d, 1H), 7.96 (s, 1H), 7.65 (s, 1H), 7.58 (d, 2H), 7.42 (d, 2H). 5.53 (s, 1H), 2.48-2.42 (m, 2H), 2.32 (m, 2H), 1.96 (tq, 1H), 1.62-1.79 (m, 1H).
storage
Store at RT
References
[1] Organic Process Research and Development, 2018, vol. 22, # 6, p. 681 - 696
[2] Patent: US2013/267493, 2013, A1. Location in patent: Paragraph 0696; 0703
[3] Journal of Medicinal Chemistry, 2016, vol. 59, # 17, p. 8068 - 8081
[4] Journal of Medicinal Chemistry, 2018, vol. 61, # 6, p. 2372 - 2383
6-chloro-5-(4-(1-hydroxycyclobutyl)phenyl)-1H-indole-3-carboxylicacid Preparation Products And Raw materials
Raw materials
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