ML-7 HYDROCHLORIDE Chemical Properties

Melting point:

246-249°C dec.

storage temp. 

-20°C

solubility 

insoluble in EtOH; ≥15.95 mg/mL in DMSO; ≥8.82 mg/mL in H2O with gentle warming and ultrasonic

form 

powder

color 

white

Safety Information

WGK Germany 

3

MSDS

• Language:English Provider:SigmaAldrich

ML-7 HYDROCHLORIDE Usage And Synthesis

Description

ML-7 inhibits smooth muscle myosin light chain kinase (MLCK) with a K_i value of 0.3 μM and displays reversible, ATP-competitive inhibition of Ca²⁺-calmodulin-dependent and -independent smooth muscle MLCKs. It exhibits a 10-fold more potent inhibition of MLCK than its parent compound ML-9 .

Chemical Properties

Off-White to Yellow Fine Crystalline Solid

Uses

A potent and selective inhibitor of myosin light chain kinase.

Uses

ML-7 has been used as a myosin light chain kinase (MLCK) inhibitor in various experiments.

Biochem/physiol Actions

Selective myosin light chain kinase inhibitor.

in vitro

rats with myocardial infarction were intravenously infused with rhnrg-1. the cmlck expression and phosphorylated mlc-2v were up-regulated in rat treated with rhnrg-1 significantly. moreover, the restoration of rhnrg-1-induced sarcomeric organization in serum-free cultured neonatal rat cardiomyocytes with rhnrg-1 was inhibited by ml-7 [1].

in vivo

administration of ml-7 from 10 min before ischemia to the first 10 min of reperfusion led to a significant recovery of heart contractility. gel analyses of two-dimensional electrophoresis revealed eight proteins with decreased levels in i/r hearts. six proteins involved in energy metabolism, which were cytochrome b-c1 complex subunit 1, atp synthase beta subunit, cytochrome c oxidase subunit, mitochondrial nadhdehydrogenase, nadhdehydrogenase iron-sulfur protein 8, and succinyl-coa ligase subunit. the other two protein levels decreased in i/r hearts, which were peroxiredoxin-2 and tubulin. in addition, ml-7 treatment increased the level of succinyl-coa ligase, which was a key enzyme involved in the citric acid cycle [2].

storage

room temperature (desiccate)

References

[1] gu x,liu x,xu d,li x,yan m,qi y,yan w,wang w,pan j,xu y,xi b,cheng l,jia j,wang k,ge j,zhou m. cardiac functional improvement in rats with myocardial infarction by up-regulating cardiac myosin light chain kinase with neuregulin. cardiovasc res.2010 nov 1;88(2):334-43.
[2] lin hb,cadete vj,sawicka j,wozniak m,sawicki g. effect of the myosin light chain kinase inhibitor ml-7 on the proteome of hearts subjected to ischemia-reperfusion injury. j proteomics.2012 sep 18;75(17):5386-95.

ML-7 HYDROCHLORIDE(110448-33-4)Related Product Information

• ML365
• ML224
• 5-[6-(4-Methoxyphenyl)pyrazolo[1,5-a]
• ML281
• ML234
• ML 141
• M348
• ML-098
• ML277
• ML329
• ML364
• ML-323
• ML264
• ML216
• Phosphorus pentachloride
• ARSENIC PENTASULPHIDE
• Hexane
• CID-49830258